Zymogram studies of human intestinal brush border and cytoplasmic peptidases.
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چکیده
منابع مشابه
Zymogram studies of human intestinal brush border and cytoplasmic peptidases.
Zymogram studies of peptide hydrolases from the human intestinal brush border and cytoplasmic fractions produced multiple bands--that is, up to seven--while the brush border membrane produced only a single band of enzyme activity. With all of the substrates tested except L-leucyl-L-leucyl-L-leucine, a band having anodic mobility identical with that produced by the brush border enzymes was produ...
متن کاملRat Intestinal Brush Border Membrane Peptidases
The properties of two purified peptidases derived from the intestinal brush border membrane of the rat have been investigated. The pH optima, heat stabilities, substrate specificities, and metal ion requirements of the two enzymes and the effects of inhibitors on their activities were nearly identical. The isoenzymes catalyzed the hydrolysis of a wide range of peptides containing from 2 to 8 am...
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SCOPE This study aimed to assess the impact of the "often neglected" intestinal brush border membranes (BBMs) hydrolases on dietary peptides, exploring the possibility that the disintegration of proteins progressed in the small intestine up to a "core" of intrinsically stable oligopeptides, persisting independently on the up-stream breakdown. METHODS AND RESULTS Samples of sodium caseinate, s...
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Epithelial cells from diverse tissues, including the enterocytes that line the intestinal tract, remodel their apical surface during differentiation to form a brush border: an array of actin-supported membrane protrusions known as microvilli that increases the functional capacity of the tissue. Although our understanding of how epithelial cells assemble, stabilize, and organize apical microvill...
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The surface membrane of the small intestine comes into contact with luminal nutrients, organisms, drugs and foreign antigens. The natural frontier between the body and the intestinal content is represented by the glycoproteins of the brush border membrane, which act as receptors, carriers and hydrolytic enzymes. Developmental variability of these glycoproteins may therefore regulate intestinal ...
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ژورنال
عنوان ژورنال: Gut
سال: 1979
ISSN: 0017-5749
DOI: 10.1136/gut.20.11.987